EGFP expression in the midbrain (neurons, MHB), hindbrain (neurons, ventricle), spinal cord (neurons), eyes, ear and ionocytes.

Genomic location: Chromosome 14

The insertion is in the intron of LOC560564 (similar to spinal cord-derived growth factor)

DNA sequences flanking insertion site

5:CCTAAACACATGCTCAGAAATGGCGTAATTACTTCAAAATTAAATACTGA

AAGACATTTATATTGGTCGACAACTAAACCCAATATTTAGGGAGGCTGCATT

TAAGGGGTCTGCTCTTTGGTTAATACCAGCTCTTCCCACAGGAGAGGGGAAT

GCAGCAGTCTCTCTTTGCTTTTAATTATGACAGACACTTGTTGCATATAAAT

TATCCTGGAGCAAGAGAAGCCATCTCTGATCATCAGGGTCATGACAAGGTGA

AATAAAACACATCTGTAGTATTTTTTGTCAGTCCCTCGCTCAAACACAAATT

TGAGCATGTTGCCCAAGGACTTGATATTCATAGCAATGCTTAGCCGTGCAGC

TGCAATCTGTGTTTGTGTATACGTGTTTGATTCCTGAAAGAAGCTTAAAGTT

TTATTCTCTTATGGAAAATAAGTGGGTGATTTTAAAAAGTAATACTAAAACC

ATTGCCTCAAATGTTAATGTAATTCCTTTGTATGAAGTAATTTTTAAGGAAG

CACACAGCTATAGTTCCAGACAAATTTAACATGTTACAGTCGCATGATTTCA

GTTATTTTAATGATTCTTACAGTTTACTACAGTGGCCGAGAGAGCTGCAATT

TAAGAAAGCACTTGCAATTACAAAAAACAAATTAAGAAACGATCTTGATCAA

TTTAACAGCACACGTGGTACAAATTG

3:TGTTTAGGCTTGTCTTTCTTTCAAAAGACTCTGGAAGGACATAAATAATG

AACAAGGATACATATGACAAAAATAGAGTGCCAGCTTTACATCCATCATTTA

CAACTTTTTTTTTTTTTTT